Genomic dna was extracted from the flukes and pcrrflp was used to. Molecular characterization of fasciola hepatica from sardinia. There is fever, loss of appetite, abdominal pain, liver tenderness, jaundice and sometimes cirrhosis of the liver. Coupled with part of a traditional sedimentation technique, this approach presents a new molecular diagnostic workflow capable of the specific detection of f. Fasciola hepatica is found on every continent with nearly 180 million people at risk and an estimated 2. This species requires complex intermediate stages to complete its life cycle. Molecular and morphological characterization of fasciola.
Infections in local breeds of sheep in middle awash river basin, afar region, ethiopia endris feki ahmed1, kanchana markvichitr2, sornthep tumwasorn2, skorn koonawootrittriron 2,apassara choothesa3 and sathaporn jitapalapong 4 abstract. Fascioliasis is caused by fasciola hepatica and fasciola gigantica. Molecular characterization of fasciola species isolated from. Pdf documents are often used because they help preserve the original content of the document, but this can make splitting the file up a little more difficult than other document formats. Get a printable copy pdf file of the complete article 2.
Biology, prevention and control of paramphistomum spp, the rumen fluke, parasite of cattle, sheep, goats and other livestock. Both species infect the liver tissue of a wide variety of mammals, including humans, in a condition known as fascioliasis. Fasciola hepatica, also known as the common liver fluke or sheep liver fluke, is a parasitic trematode fluke or flatworm, a type of helminth of the class trematoda, phylum platyhelminthes. Genetic characterization, species differentiation and. Refer to the help section for more detailed instructions. Fasciola, commonly known as the liver fluke, is a genus of parasitic trematodes. Most downloaded international journal for parasitology articles the most downloaded articles from international journal for parasitology in the last 90 days. Mf6pfhhdm1 is a small cationic hemebinding protein which is recognized by the monoclonal antibody mab mf6, and abundantly present in parenchymal cells and secreted antigens of fasciola hepatica.
Fascioliasis, an infection due to the food and waterborne trematodes fasciola hepatica and fasciola gigantica, is among the most neglected of the neglected tropical diseases. Fasciola hepatica, or common liver fluke, is the agent of fascioliasis, a disease mainly affecting ruminants and more rarely humans. Host responses during experimental infection with fasciola. Molecular characterization of fasciola hepatica from sardinia based on sequence analysis of genomic and mitochondrial gene markers sarra farjallaha, badreddine ben slimanea, cristina maria pirasb, nabil amorc. Fascioliasis is a parasitic infection typically caused by fasciola hepatica, which is also known as. They are surrounded by a frothy bronchial exudate and they cause atelectasis and emphysema secondary to the bronchitis, and which is provoked by the eggs and larvae.
It is important to get an idea about the fasciola hepatica life cycle to determine the best treatment option. Shafiei3 1infectious disease and tropical medicine research center, zahedan university of. Most downloaded international journal for parasitology. Fayez muhammad shaldoum, alaaabd elaziz muhammad, adham gamal sadek, mohamed koraim yassin, ali osman elmadawy and ibraheem mostafa gobaara. Materials and methods fluke collection and the isolation of genomic dna we chose to study several different regions in pakistan, where we anticipated fasciola spp. Fascioliasis definition of fascioliasis by medical dictionary. Right now, a generous supporter will match your donation 2to. Both fasciola hepatica and fasciola gigantica are known to occur in cattle. Pdf fasciolosis is a zoonosis affecting ruminants, caused by the liver flukes fasciola f. Protein concentration was measured by bradford method and sods band protein was detected on sdspage. Little is known on the identity and genetic variability of fasciola populations in sheep. Furthermore, fasciolosis was significantly more prevalent than dicrocoeliosis in both sheep. A related parasite, fasciola gigantica, also can infect people.
Gilleard1 1department of comparative biology and experimental medicine, faculty of veterinary medicine, university of calgary, alberta, canada. Split pdf file separate one page or a whole set for easy conversion into independent pdf files. Table 2 shows the global number of paragonimus spp. Molecular characterization of fasciola hepatica from. Saifullah3, raman muthusamy4, ravikumar gopalakrishnan4, terry w. Fasciola hepatica sheep liver fluke phylum platyhelminthe class trematoda fasciola gigantica very rarely infects humans. Phylogenetic analysis of fasciola species is critical because of their different epidemiology. A portrait of the transcriptome of the neglected trematode, fasciola giganticabiological and biotechnological implications. The trematodes fasciola hepatica also known as the common liver fluke or the sheep liver fluke and fasciola gigantica are large liver flukes f. Fascioliasis is a foodborne parasitic disease caused by the trematode species fasciola hepatica, distributed worldwide, and fasciola gigantica, restricted to given regions of africa and asia. Both fasciola hepatica and fasciola gigantica are known to occur in cattle, providing the opportunity for genetic. Infections in local breeds of sheep in middle awash river basin, afar region, ethiopia endris feki ahmed1, kanchana markvichitr2, sornthep tumwasorn2, skorn koonawootrittriron 2,apassara choothesa3 and sathaporn jitapalapong 4. Fascioliasis is a parasitic infection typically caused by fasciola hepatica, which is also known as the common liver fluke or the sheep liver fluke.
Archives of clinical infectious diseases identification of. The identity and genetic variability of fasciola spp. Prevalence is highest in areas where extensive sheep and cattle raising occurs and where dietary practices include the consumption of raw aquatic vegetables. In sheep, the migratory phase of the parasite produces severe parenchymal disease of the liver for which the parasite is called sheep liver fluke and the disease is known as liver rot. The disease is a plantborne trematode zoonosis, and is classified as a neglected tropical disease ntd. Pdf educational material find, read and cite all the research you need on researchgate. The lung lesions appear as fairly large wedgeshaped areas, dark red or grey in colour, usually.
Fasciola is a genus of trematodes that comprises species of economic importance. Fascioliasis is found in all continents except antarctica, in over 70 countries, especially where there are sheep or cattle. It affects humans, but its main host is ruminants such as cattle and sheep. Fasciolidae, have a serious impact on the farming industry and public health.
Orthologs of this protein mf6phdms also exist in other causal agents of important foodborne trematodiasis, such as clonorchis sinensis, opisthorchis viverrini and paragonimus westermani. Apr, 2020 fasciola hepatica can affect 46 species of wild and domestic animals as well as humans. Fasciola gigantica an overview sciencedirect topics. Original article molecular and phylogenetic characterisation.
Parasitological and hematological study on fasciola spp. What links here related changes upload file special pages permanent link page information wikidata. Fasciola is a genus of trematodes which includes economically important parasite species. The current study aimed to find out the morphometric and genotypic divergences of the flukes isolated from different hosts in a newly emerging focus of human fascioliasis in iran. Simple morphological criteria are unreliable for their specific identification. For this to be of benefit, animals should not be placed on clean pasture until 6 hours. We have developed a restriction fragment length polymorphism of amplified dna pcrrflp of the nuclear ribosomal internal transcribed. To screen a large number of animals for the presence of fasciola spp. Characterization of fasciola gigantica isolates from. Select up to three search categories and corresponding keywords using the fields to the right.
The current study aimed at identifying fasciola spp. The infection caused by trematode parasites of the genus fasciola can infect. Fasciola parasites live as adult flukes in the bile duct and immature eggs go out through feces and grow in fresh. Fascioliasis due to fasciola hepatica and fasciola gigantica. Original article molecular and phylogenetic characterisation of fasciola spp. The results revealed that the prevalence rate of fasciola spp. Early pathological changes associated with fasciola hepatica. In this study, experiments were carried out for the first trials to show antiparasitic activity of calligonum comosum in the treatment of fasciola spp. Molecular and morphological characterization of fasciola spp. Fasciola hepatica life cycle pdf immunology medical specialties. Which species is in the faeces at a time of global.
Liver flukes reveals the persistence of gene silencing in vitro paul mcveigh1, erin m. Fasciola hepatica, also known as the common liver fluke or sheep liver fluke, is a parasitic. Fasciolidae in egypt molecular identification of fasciola spp. Helminth infections of ruminants 3 p a g e infections of the lungs and trachea dictyocaulus filaria is the lungworm that occurs in the trachea and bronchi of sheep and goats worldwide. Fasciola hepatica life cycle pdf free download as pdf file. Free fulltext pdf articles from hundreds of disciplines, all in one place. Several molecular studies have been conducted in north and south of iran for genotype analysis of fasciola spp. Patterns in size and shedding of fasciola hepatica eggs by naturally and experimentally infected murid rodents pdf. Hybridization between the two fasciola species has been documented, leading to the emergence of intermediate forms with mixed phenotypic characteristics and genetic structure 15.
Economically important infections are seen in cattle, sheep, alpacas, and llamas in three forms. Molecular characterization is important for discriminating fasciola specimens having the deoxyribonucleic acid dna sequences of fasciola hepatica, fasciola gigantica, and both fasciola species, since three fasciola forms coexist in asian countries. This disease in humans shows an increasing importance, which relies on its recent widespread emergence related to climate and global changes and also on. To the internet archive community, time is running out. Species of fasciola are commonly known as liver flukes. Fasciola species are the main causes for fascioliasis with great financial losses and are among the most important foodwaterborne parasites worldwide. Oct 20, 2014 the two species of trematodes that cause fascioliasis fasciola hepatica and fasciola gigantica are leafshaped worms, large enough to be visible to the naked eye adult f. Rearrange individual pages or entire files in the desired order. The disease caused by the fluke is called fasciolosis or fascioliasis, which is a type of helminthiasis and has been classified as a neglected. The common liver fluke fasciola hepaticathis large worm inhabits the liver of the goat and other herbivores. Both fasciola hepatica and fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. It infects the livers of various mammals, including humans. Molecular characterization of fasciola species isolated. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Fasciola hepatica commonly called sheep liver fluke. In previous studies, promising phenotypic identification scores were obtained using. Archives of clinical infectious diseases identification. Ostertagia teladorsagia spp trichostrongylus spp cooperia spp oesophagostomum columbianum nematodirus spp oesophagostomum venulosum chabertia ovina dictyocaulus. The life cycle is direct and the developmental period is 2830 days. Helminth infections of ruminants 4 p a g e easy to see. Fascioliasis, fasciola hepatica, fasciola gigantica, trematodiasis, neglected.
Besides causing liver condemnations, high mortality rate, reduced production of meat and milk in the cattle industry. Nielsen, in advances in food and nutrition research, 2017. Dicrocoeliosis was more dominant in female animals 7. Fascioliasis definition of fascioliasis by medical. Fascioliasis due to fasciola hepatica and fasciola. The life cycle, transmission, morphology, clinical presentation, and treatment of the f. Evaluation of the performance of five diagnostic tests for fasciola. A portrait of the transcriptome of the neglected trematode, fasciola. Fasciola hepatica can affect 46 species of wild and domestic animals as well as humans. Fasciola hepatica bahiya basheer 2nd year mbbs student 2. Superoxide dismutase sod enzyme activity assay in fasciola. Bcs were randomly selected for collection of feces and screened for presence of fasciola eggs using fecal sedimentation test.
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